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cytoskeletal reorganisation  (Cytoskeleton Inc)


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    Cytoskeleton Inc cytoskeletal reorganisation
    Paclitaxel-induced <t>cytoskeletal</t> <t>reorganisation</t> around the nucleus in interphase. (A) Confocal micrographs of cells fixed after 16 h incubation in control medium or 5 nM paclitaxel. DNA was stained using Hoechst 33342 (cyan), and microtubules using α-tubulin immunofluorescence (magenta). MTOCs are marked with arrowheads. Scale bars: 20 μm. (B) STORM imaging of α-tubulin immunofluorescence in cells fixed after 16 h incubation in control medium or 5 nM paclitaxel. Lower panels show α-tubulin clusters generated with HDBSCAN analysis. Different colours distinguish individual α-tubulin clusters, representing individual microtubule filaments or filament bundles. Scale bars: 10 μm. (C) Violin plot comparing the diameter of α-tubulin clusters from B between control (blue) and paclitaxel-treated cells (red). The mean is shown in black with error bars showing the s.e.m. from three biological repeats ( n =3). The mean of each biological repeat is marked with a triangle and consists of >300 measurements from five or more cells. *** P =0.00012 (unpaired two-tailed t -test). (D,E) Confocal micrographs of control and paclitaxel-treated cells stained for DNA using Hoechst 33342 (cyan), microtubules using α-tubulin immunofluorescence (magenta), and either (D) F-actin using Alexa Fluor 488–phalloidin (green) or (E) vimentin using immunofluorescence (green). Scale bars: 20 μm. (F) Left, 2D slice of a reconstructed tomogram of the NE region in a paclitaxel-treated cell. Bundled vimentin filaments (green arrowheads) and microtubules (magenta arrowheads) are seen closely apposed to the NE (cyan arrowheads). Right, segmentation of the NE (cyan), microtubules (magenta) and vimentin filaments (green) from the tomogram. Scale bars: 100 nm. Images in A and D–F are representative of three biological repeats each with >50 cells.
    Cytoskeletal Reorganisation, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 95/100, based on 353 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cytoskeletal+reorganisation/pmc12863304-48-4-20?v=Cytoskeleton+Inc
    Average 95 stars, based on 353 article reviews
    cytoskeletal reorganisation - by Bioz Stars, 2026-08
    95/100 stars

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    1) Product Images from "Paclitaxel compromises nuclear integrity in interphase through SUN2-mediated cytoskeletal coupling"

    Article Title: Paclitaxel compromises nuclear integrity in interphase through SUN2-mediated cytoskeletal coupling

    Journal: Journal of Cell Science

    doi: 10.1242/jcs.264494

    Paclitaxel-induced cytoskeletal reorganisation around the nucleus in interphase. (A) Confocal micrographs of cells fixed after 16 h incubation in control medium or 5 nM paclitaxel. DNA was stained using Hoechst 33342 (cyan), and microtubules using α-tubulin immunofluorescence (magenta). MTOCs are marked with arrowheads. Scale bars: 20 μm. (B) STORM imaging of α-tubulin immunofluorescence in cells fixed after 16 h incubation in control medium or 5 nM paclitaxel. Lower panels show α-tubulin clusters generated with HDBSCAN analysis. Different colours distinguish individual α-tubulin clusters, representing individual microtubule filaments or filament bundles. Scale bars: 10 μm. (C) Violin plot comparing the diameter of α-tubulin clusters from B between control (blue) and paclitaxel-treated cells (red). The mean is shown in black with error bars showing the s.e.m. from three biological repeats ( n =3). The mean of each biological repeat is marked with a triangle and consists of >300 measurements from five or more cells. *** P =0.00012 (unpaired two-tailed t -test). (D,E) Confocal micrographs of control and paclitaxel-treated cells stained for DNA using Hoechst 33342 (cyan), microtubules using α-tubulin immunofluorescence (magenta), and either (D) F-actin using Alexa Fluor 488–phalloidin (green) or (E) vimentin using immunofluorescence (green). Scale bars: 20 μm. (F) Left, 2D slice of a reconstructed tomogram of the NE region in a paclitaxel-treated cell. Bundled vimentin filaments (green arrowheads) and microtubules (magenta arrowheads) are seen closely apposed to the NE (cyan arrowheads). Right, segmentation of the NE (cyan), microtubules (magenta) and vimentin filaments (green) from the tomogram. Scale bars: 100 nm. Images in A and D–F are representative of three biological repeats each with >50 cells.
    Figure Legend Snippet: Paclitaxel-induced cytoskeletal reorganisation around the nucleus in interphase. (A) Confocal micrographs of cells fixed after 16 h incubation in control medium or 5 nM paclitaxel. DNA was stained using Hoechst 33342 (cyan), and microtubules using α-tubulin immunofluorescence (magenta). MTOCs are marked with arrowheads. Scale bars: 20 μm. (B) STORM imaging of α-tubulin immunofluorescence in cells fixed after 16 h incubation in control medium or 5 nM paclitaxel. Lower panels show α-tubulin clusters generated with HDBSCAN analysis. Different colours distinguish individual α-tubulin clusters, representing individual microtubule filaments or filament bundles. Scale bars: 10 μm. (C) Violin plot comparing the diameter of α-tubulin clusters from B between control (blue) and paclitaxel-treated cells (red). The mean is shown in black with error bars showing the s.e.m. from three biological repeats ( n =3). The mean of each biological repeat is marked with a triangle and consists of >300 measurements from five or more cells. *** P =0.00012 (unpaired two-tailed t -test). (D,E) Confocal micrographs of control and paclitaxel-treated cells stained for DNA using Hoechst 33342 (cyan), microtubules using α-tubulin immunofluorescence (magenta), and either (D) F-actin using Alexa Fluor 488–phalloidin (green) or (E) vimentin using immunofluorescence (green). Scale bars: 20 μm. (F) Left, 2D slice of a reconstructed tomogram of the NE region in a paclitaxel-treated cell. Bundled vimentin filaments (green arrowheads) and microtubules (magenta arrowheads) are seen closely apposed to the NE (cyan arrowheads). Right, segmentation of the NE (cyan), microtubules (magenta) and vimentin filaments (green) from the tomogram. Scale bars: 100 nm. Images in A and D–F are representative of three biological repeats each with >50 cells.

    Techniques Used: Incubation, Control, Staining, Immunofluorescence, Imaging, Generated, Two Tailed Test



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    cytoskeletal reorganisation  (Cytoskeleton Inc)
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    Cytoskeleton Inc cytoskeletal reorganisation
    Paclitaxel-induced <t>cytoskeletal</t> <t>reorganisation</t> around the nucleus in interphase. (A) Confocal micrographs of cells fixed after 16 h incubation in control medium or 5 nM paclitaxel. DNA was stained using Hoechst 33342 (cyan), and microtubules using α-tubulin immunofluorescence (magenta). MTOCs are marked with arrowheads. Scale bars: 20 μm. (B) STORM imaging of α-tubulin immunofluorescence in cells fixed after 16 h incubation in control medium or 5 nM paclitaxel. Lower panels show α-tubulin clusters generated with HDBSCAN analysis. Different colours distinguish individual α-tubulin clusters, representing individual microtubule filaments or filament bundles. Scale bars: 10 μm. (C) Violin plot comparing the diameter of α-tubulin clusters from B between control (blue) and paclitaxel-treated cells (red). The mean is shown in black with error bars showing the s.e.m. from three biological repeats ( n =3). The mean of each biological repeat is marked with a triangle and consists of >300 measurements from five or more cells. *** P =0.00012 (unpaired two-tailed t -test). (D,E) Confocal micrographs of control and paclitaxel-treated cells stained for DNA using Hoechst 33342 (cyan), microtubules using α-tubulin immunofluorescence (magenta), and either (D) F-actin using Alexa Fluor 488–phalloidin (green) or (E) vimentin using immunofluorescence (green). Scale bars: 20 μm. (F) Left, 2D slice of a reconstructed tomogram of the NE region in a paclitaxel-treated cell. Bundled vimentin filaments (green arrowheads) and microtubules (magenta arrowheads) are seen closely apposed to the NE (cyan arrowheads). Right, segmentation of the NE (cyan), microtubules (magenta) and vimentin filaments (green) from the tomogram. Scale bars: 100 nm. Images in A and D–F are representative of three biological repeats each with >50 cells.
    Cytoskeletal Reorganisation, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cytoskeletal+reorganisation/pmc12863304-48-4-20?v=Cytoskeleton+Inc
    Average 95 stars, based on 1 article reviews
    cytoskeletal reorganisation - by Bioz Stars, 2026-08
    95/100 stars
    		  Buy from Supplier

    Image Search Results


    Paclitaxel-induced cytoskeletal reorganisation around the nucleus in interphase. (A) Confocal micrographs of cells fixed after 16 h incubation in control medium or 5 nM paclitaxel. DNA was stained using Hoechst 33342 (cyan), and microtubules using α-tubulin immunofluorescence (magenta). MTOCs are marked with arrowheads. Scale bars: 20 μm. (B) STORM imaging of α-tubulin immunofluorescence in cells fixed after 16 h incubation in control medium or 5 nM paclitaxel. Lower panels show α-tubulin clusters generated with HDBSCAN analysis. Different colours distinguish individual α-tubulin clusters, representing individual microtubule filaments or filament bundles. Scale bars: 10 μm. (C) Violin plot comparing the diameter of α-tubulin clusters from B between control (blue) and paclitaxel-treated cells (red). The mean is shown in black with error bars showing the s.e.m. from three biological repeats ( n =3). The mean of each biological repeat is marked with a triangle and consists of >300 measurements from five or more cells. *** P =0.00012 (unpaired two-tailed t -test). (D,E) Confocal micrographs of control and paclitaxel-treated cells stained for DNA using Hoechst 33342 (cyan), microtubules using α-tubulin immunofluorescence (magenta), and either (D) F-actin using Alexa Fluor 488–phalloidin (green) or (E) vimentin using immunofluorescence (green). Scale bars: 20 μm. (F) Left, 2D slice of a reconstructed tomogram of the NE region in a paclitaxel-treated cell. Bundled vimentin filaments (green arrowheads) and microtubules (magenta arrowheads) are seen closely apposed to the NE (cyan arrowheads). Right, segmentation of the NE (cyan), microtubules (magenta) and vimentin filaments (green) from the tomogram. Scale bars: 100 nm. Images in A and D–F are representative of three biological repeats each with >50 cells.

    Journal: Journal of Cell Science

    Article Title: Paclitaxel compromises nuclear integrity in interphase through SUN2-mediated cytoskeletal coupling

    doi: 10.1242/jcs.264494

    Figure Lengend Snippet: Paclitaxel-induced cytoskeletal reorganisation around the nucleus in interphase. (A) Confocal micrographs of cells fixed after 16 h incubation in control medium or 5 nM paclitaxel. DNA was stained using Hoechst 33342 (cyan), and microtubules using α-tubulin immunofluorescence (magenta). MTOCs are marked with arrowheads. Scale bars: 20 μm. (B) STORM imaging of α-tubulin immunofluorescence in cells fixed after 16 h incubation in control medium or 5 nM paclitaxel. Lower panels show α-tubulin clusters generated with HDBSCAN analysis. Different colours distinguish individual α-tubulin clusters, representing individual microtubule filaments or filament bundles. Scale bars: 10 μm. (C) Violin plot comparing the diameter of α-tubulin clusters from B between control (blue) and paclitaxel-treated cells (red). The mean is shown in black with error bars showing the s.e.m. from three biological repeats ( n =3). The mean of each biological repeat is marked with a triangle and consists of >300 measurements from five or more cells. *** P =0.00012 (unpaired two-tailed t -test). (D,E) Confocal micrographs of control and paclitaxel-treated cells stained for DNA using Hoechst 33342 (cyan), microtubules using α-tubulin immunofluorescence (magenta), and either (D) F-actin using Alexa Fluor 488–phalloidin (green) or (E) vimentin using immunofluorescence (green). Scale bars: 20 μm. (F) Left, 2D slice of a reconstructed tomogram of the NE region in a paclitaxel-treated cell. Bundled vimentin filaments (green arrowheads) and microtubules (magenta arrowheads) are seen closely apposed to the NE (cyan arrowheads). Right, segmentation of the NE (cyan), microtubules (magenta) and vimentin filaments (green) from the tomogram. Scale bars: 100 nm. Images in A and D–F are representative of three biological repeats each with >50 cells.

    Article Snippet: The extent of the cytoskeletal reorganisation correlated with the concentration of paclitaxel, as microtubules became increasingly bundled and the actin cytoskeleton more condensed with increasing concentrations (0 to 10 nM) of paclitaxel ( ).

    Techniques: Incubation, Control, Staining, Immunofluorescence, Imaging, Generated, Two Tailed Test